哈维氏弧菌TS-628菌株趋化性研究

Chemotaxis of Vibrio harveyi TS-628

关键词:  趋化性  哈维氏弧菌  青石斑

Key words:  hemotaxis  V. harveyi  E. awoara

摘要:

采用平板趋化和毛细管趋化的方法，研究了病原性哈维氏弧菌TS-628菌株的趋化性。哈维氏弧菌对青石斑肌肉、表皮黏液、肠黏液及血清的趋化性研究结果表明，该菌对青石斑肌肉提取物的趋化性最强，且哈维氏弧菌的趋化运动明显受到温度、pH及盐度等环境因子的影响。哈维氏弧菌对青石斑肌肉18种主要氨基酸的趋化性研究表明，该菌对其中多种氨基酸均表现出很强的趋化性，但氨基酸浓度也会对该菌的趋化性造成影响，浓度过高趋化性反而减弱。

Abstract:

In this paper, chemotaxis of Vibrio harveyi TS-628 strain was studied by drop assay and capillary assay. The study of chemotaxis of V.harveyi to muscle extract, cutaneous mucus, intestinal mucus, serum of Epinephelus awoara showed that V. harveyi displayed stronger chemotaxis to muscle extract than to other tissues and the chemotaxis of V. harveyi was remarkably affected by temperature, pH and NaCl concentration. Furthermore, chemotaxis of V. harveyi to 18 kinds of amino acid of E. awoara muscle was studied, the results indicated that V. harveyi had strong chemotaxis to many kinds of amino acid and the most attractive amino acid was Val. However，the concentration of amino acid also affected the chemotaxis of V. harveyi.