不同地理群体星突江鲽养殖子一代群体遗传多样性分析

Genetic diversity analysis of the first filial generation of different cultured population of Platichthys stellatus

关键词:  星突江鲽  遗传多样性  养殖群体

Key words:  Starry flounder Platichthys stellatus  Genetic diversity  Cultured population

摘要:

采用RAPD标记技术对取自韩国沿海和俄罗斯沿海的星突江鲽养殖子一代进行了遗传多样性分析。从40个10bp随机引物中筛选出11个效果稳定的引物，对每个群体40个个体进行扩增，各获得57个位点。韩国群体多态位点数为33个，其多态位点比例P为57.89%；俄罗斯群体多态位点数为32个，其多态位点比例P为56.14%。韩国群体和俄罗斯群体的Shannon遗传多样度分别为0.3384和0.2963， Nei的多样性指数h分别为0.2305和0.194 7。星突江鲽两群体遗传多态度总量Hsp为0.425 2。其中，群体内遗传多态度Hpop为0.3174，源于群体内的遗传多样性的比例为0.7465，而源于群体间的遗传多样性的比例为0.2535。

Abstract:

Genetic diversity of the first filial generation of two cultured populations of Platichthys stellatus from South Korea (S) and Russia (R) was examined. Eleven from 40 random primers were used for population genetic analysis, and a total of 57 clear RAPD bands ranging from 300 to 2500 bp were recognized. The numbers of polymorphic bands of S and R populations were 33 and 32, respectively, and the mean percentages of polymorphic bands of them were 57.89% and 56.14%, Nei's diversity index of them were 0.230 5 and 0.194 7, and Shannon information index of them were 0.338 4 and 0.296 3. Partitioning analysis of genetic diversity in Shannon index indicated that 74.65% of genetic variation was distributed within populations and 25.35% was between populations.