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| 大珠母贝两个野生群体遗传多样性的微卫星分析 |
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谷龙春1, 黄桂菊2, 何毛贤3, 喻达辉2
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1.(1中国水产科学研究院南海水产研究所,广州 510300)
(2上海海洋大学生命学院,201306);2.(1中国水产科学研究院南海水产研究所,广州 510300);3.(3中国科学院南海海洋研究所,广州 510301)
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| 摘要: |
| 利用6对微卫星DNA分子标记对三亚和北海的大珠母贝群体进行遗传多样性分析。结果表明，两个群体的平均等位基因数A分别为10.5和9.7，平均有效等位基因数Ne为7.0和6.3，平均观察杂合度Ho为0.588和0.445，平均期望杂合度He为0.859和0.828，平均多态信息含量PIC为0.853和0.796；卡方检验发现只有位点Pmx+022、Pmx16_41和Pmx16_23在三亚种群中Hardy-Weinberg平衡偏离不显著(P＞0.05)，其他位点在两个种群都有不同程度的偏离。 |
| 关键词: 大珠母贝 野生群体 遗传多样性 微卫星DNA |
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| 基金项目:国家高技术研究发展计划（863）项目（2006AA10A415）和广东省科技兴海项目（A200701C02）共同资助 |
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| Genetic diversity of two wild populations of pearl oysterPinctada maximaas revealed by microsatellite DNA |
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| Abstract: |
| Genetic variation between wild populations of Pinctada maxima from Sanya and Beihai was assessed by microsatellite DNA. The result showed that average number of alleles (A) was 10.50 and 9.67, and average number of effective alleles (Ne) was 7.01 and 6.33, respectively. The average observed heterozygosity (Ho) was 0.588 and 0.445, and the expected average heterozygosity (He) was 0859 and 0828, respectively. The average polymorphism information content (PIC) was 0.853 and 0.796, respectively. Hardy Weinberg equilibrium analysis revealed that these two populations showed some genetic disequilibria at the most loci except the loci of Pmx+022, Pmx16_41 and Pmx16_23 in Sanya population. |
| Key words: Pinctada maxima Wild population Genetic diversity Microsatellite DNA |