长鳍真鲨源哈氏弧菌的主要生物学性状研究

Biological characteristics of Vibrio harveyi from diseased oceanic whitetip shark Carcharhinus longimanus L.

关键词:  长鳍真鲨  哈氏弧菌  生物学性状  系统发育学

Key words:  Oceanic whitetip shark （Carcharhinus longimanus L.）  Vibrio harveyi  Biological  Characterization

摘要:

从两尾病死的观赏用长鳍真鲨Oceanic whitetip shark，Carcharhinus longimanus L.中分离到的细菌，进行了形态特征、理化特性和对抗菌类药物的敏感性等生物学性状检验。同时，测定了16S rRNA基因序列，分析了相关细菌相应序列的同源性，构建了系统发育树。结果表明，8株供试纯培养菌（编号：HQ050226-1至HQ050226-4、HQ050227-1至HQ050227-4）为弧菌属（Vibrio Pacini 1854）的哈氏弧菌（Vibrio harveyi Johnson and Shunk 1936）。所扩增的16S rRNA基因序列长度（不包括引物结合区），HQ050226-1株为1463bp（GenBank 登录号：EF635305）、HQ050227-1株为1464bp（GenBank 登录号：EF635306），与GenBank数据库中哈氏弧菌的同源性在98%及99%。药敏试验结果显示，对供试37种抗菌药物中的青霉素G等4种耐药, 对头孢唑啉等33种敏感。

Abstract:

The biological characteristics of bacteria isolated from oceanic whitetip shark Carcharhinus longimanus L. were examined, including morphological characteristics, physiological and biochemical characteristics and antibiotic sensitivity. In addition, the 16S rRNA gene were partially sequenced and compared with sequences listed in databases; molecular phylogenetic trees were constructed. The results showed that the eight pure strains（HQ050226-1 to HQ050226-4 and HQ050227-1 to HQ050227-4）belonged to Vibrios (V. harveyi). The sequenced 16S rRNA gene of strain HQ050226-1 (GenBank accession No. EF635305) was 1463bp in length, the sequenced 16S rRNA gene of strain HQ050227-1 (GenBank accession No. EF635306) was 1464bp in length, and exhibited high similarity (98% and 99%) to the 16S rRNA gene of V. harveyi from GenBank database. Antibiotic sensitivity using 37 antimicrobial agents showed that isolates were resistant to 4 agents including penicillin G，but were sensitive to 33 agents including cefazolin.