Cloning and Expression Analysis of EcERR in Exopalaemon carinicauda

Abstract:

Estrogen-related receptor (ERR) is a eukaryotic transcription factor that is closely related to the effect of estrogen and is involved in the regulation of energy metabolism, cell proliferation and differentiation, gonadal development, and other physiological processes. To understand the role of estrogen-related receptors in the ovarian development of Exopalaemon carinicauda, the EcERR was cloned using RACE technology. The gene is 2025 bp long, including an open reading frame of 1374 bp, a 5′ untranslated region of 241 bp, and a 3′ untranslated region of 410 bp, which encodes 457 amino acids. Tissue expression analysis showed that EcERR was expressed in many E. carinicauda tissues, with the highest expression level in ovarian tissue. The expression level of EcERR in the cleavage stage was significantly higher than in that of other embryo and larval development stages (P<0.05). The expression level of EcERR first decreased and then increased during ovarian development, with the highest expression level in StageⅠ. After 5-hydroxytryptamine invaded, the expression levels of vitellogenin and EcERR in the experimental group were significantly higher than in the control group (P<0.05). The results showed that EcERR is involved in the regulation of E. carinicauda ovarian development and plays a key role in its early stages.