Genetic structure analysis of different populations of spotted sea bass (Lateolabrax maculatus)

Abstract:

The 86 spotted sea bass (Lateolabrax maculatus) used as experimental subjects were collected from eight main cultured populations, namely Shandong Province (Qingdao and Dongying), Zhejiang Province (Ningbo), Fujian Province (Fuding, Meiling Town of Zhangzhou, Qiaodong Town of Zhangzhou, and Dongshan Town of Zhangzhou), and Guangdong (Doumen District of Zhuhai). Based on the reported genetic background of wild populations of spotted sea bass, we conducted a genetic structure analysis on the eight cultured populations using the double-digest restriction-site-associated DNA tag sequencing and simplified genome sequencing analysis technology. The results of a principal component analysis and genetic ancestry analysis (the best clustering K value is 2) showed that there was little genetic divergence between the eight cultured populations and the wild populations of Tianjin, Yantai, and Wendeng in Shandong Province, indicating that the germplasm sources for cultured spotted sea bass are mainly the Yellow Sea and Bohai Sea. The results of the genetic differentiation analysis among the eight cultured populations showed that there was significant genetic divergence between the Meiling Town and Qiaodong Town populations in Zhangzhou and other cultured populations, indicating that a certain degree of differentiation appeared within the identified cultured populations. The results provide a reference for the analysis of the genetic structure of cultured spotted sea bass populations from Shandong to Guangdong (north to south), and provide a basis for the protection and improvement of spotted sea bass germplasm resources and breeding of spotted sea bass.