Cloning, expression of hsp70 core cDNA and the effects of heat stress on its tissue expression in Pelteobagrus fulvidraco ♀×P. vachelli ♂

Abstract:

Heat shock protein 70 (HSP70) is closely related to the stress resistance and anti-stress ability of organisms, where it plays a crucial role. In the present study, a 735 bp core cDNA sequence of the hsp70 gene isolated from the liver tissue of Pelteobagrus fulvidraco ♀×P. vachelli ♂ was amplified using reverse transcription polymerase chain reaction (RT-PCR), and the bioinformatics was analyzed. The relative mRNA expression of the hsp70 gene in different tissues was tested using quantitative real-time PCR (qPCR), which revealed that hsp70 mRNA was found in all tested tissues, including liver, gill, brain, and muscle, and hsp70 mRNA expression levels in liver tissue were significantly different from that in brain, gill, and muscle tissue (P<0.05). At 20℃ (control), 25℃, 28℃, and 31℃, the hsp70 gene expression in liver, gill, brain, muscle tissue generally increased with increasing temperature. The hsp70 gene expression level in gill tissue at 28℃ and 31℃ was significantly higher than that in other tissues (P<0.05), which indicated that gill tissue was sensitive to heat stress in the hybrid. It was suggested that this may be an organism protection strategy for gill tissue, which is important for respiratory metabolism under extreme changes in temperature.