Development of SNP markers and verification analysis of relationship on family in Litopenaeus vannamei

Abstract:

The Large-scale family-based selective breeding can effectively increase genetic gain in shrimp, but tagging individuals for tracking pedigree is time-consuming, labor-intensive, and would cause inaccurate kinship. Molecular markers are effective in solving this problem. In this study, we developed 37 SNP markers derived from transcriptome sequences and performed analysis on genetic diversity, family clustering, and parentage for 22 families of Litopenaeus vannamei. The results provided examples for the application of SNP markers in selective breeding of shrimp. The results showed that the average expected heterozygosity (He) and observed heterozygosity (Ho) for 37 SNP loci were 0.38 and 0.34, respectively. Further, the average polymorphism information content (PIC) was 0.30, which means moderate polymorphism (0.25