Genetic Diversity Analysis of Domestic Commercial Brands Seedlings of Litopenaeus vannamei Based on Microsatellite Molecular Markers

Abstract:

To clarify the genetic diversity characteristics of domestic commercial brand seedlings of Litopenaeus vannamei, six domestic commercial brands of L. vannamei, namely, Huanghua R, Dongying M, Guangzhou P, Guangzhou Z, Hainan S, and Hainan Z, were collected from Hebei, Shandong, Guangdong, and Hainan Province. Their genetic diversity was detected using eight microsatellite markers. The results showed that the average allele number (Na), expected heterozygosity (He), observed heterozygosity (Ho), and polymorphic information content (PIC) were 4.5~9.5, 0.516~0.733, 0.346~0.550, and 0.472~0.700, respectively. The genetic diversity of the six brands ranged from high to low: Huanghua R > Guangzhou Z > Guangzhou P > Hainan Z >Dongying M > Hainan S, and 48 Hardy-Weinberg equilibrium tests were conducted on the 6 brands of L. vannamei using 8 microsatellite loci. It was observed that, 4.17% (2/48) showed significant deviation (0.01