Abstract:Sepioteuthis lessoniana is a common species of cephalopods. In order to understand the nutritive composition of its muscle, we measured and analyzed the general nutrients; amino acids, fatty acids, mineral elements, and vitamins in muscles by biochemical methods. The results showed that the contents of water, crude protein, crude fat, ash, and total sugar in the wet muscles were 76.10%, 19.01%, 1.20%, 1.71%, and 1.98%, respectively. A total of 18 amino acids were detected in muscle, the total amino acids (TAA) accounted for 64.65% of the dry samples. The content of essential amino acid (EAA) reached 22.97%, and the ratio of essential amino acids to total amino acids and total nonessential amino acids (NEAA) was 35.53% and 69.42%, respectively. The amino acid composition was considered suitable. The level of four flavor amino acids was 25.02%, and the flavor was delicious. According to the amino acid score (AAS) and chemical score (CS), the first limiting amino acid was Cystine plus Methionine, and the essential amino acid index (EAAI) was 68.02. The amino acid composition was relatively balanced. The contents of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) were 43.02%, 6.59% and 42.39%, respectively. The content of PUFA was relatively high, of which the content of EPA plus DHA reached 32.64%, this has a high health value to the human body. In addition, the muscles of S. lessoniana were rich in mineral elements such as K, Zn, and Se, and vitamins such as VB6 and VB3. In conclusion, S. lessoniana was shown to be a high-quality aquatic product with a comprehensive nutrient composition and a high nutritional value.