Genetic Background Analysis of Six Groups of White Shrimp Litopenaeus vannamei in China Using SSR Markers

Abstract:

Controlling the inbreeding level is an important task in animal breeding. Genetic analysis such as genetic diversity and genetic distance estimate could provide information for the artificial selection of animals. In the present study, the genetic information of Pacific white shrimp Litopenaeus vannamei from six different populations in China were analyzed using genotyping data of eight SSR loci. The results showed that the mean Na ranged from 3.67 to 9.33, the mean Ne range from 2.20 to 5.67, the mean Ho ranged from 0.12 to 0.71, the mean He ranged from 0.41 to 0.81, and the mean PIC ranged from 0.36 to 0.76. The cluster analysis results showed that the individual shrimps from populations of different companies were clustered in different nodes. The individual shrimps from three hatcheries from the same company were clustered in one node. Among the six populations of L. vannamei, the genetic identity ranged from 0.4229 to 0.8265, and the genetic distance ranged from 0.1905 to 0.8607. The mean value of FST was 0.1837. The FIS value was 0.2514. And the Ho value was smaller than the He value. The results suggested that genetic diversity between L. vannamei from different populations could be high, while, genetic relationship of L. vannamei in populations of the same company might be quite close. There might be an inbreeding phenomenon.