Effects of LED spectra on morphological character and gene expression of growth in Takifugu rubripes larvae

Abstract:

Four LED spectral parameters were set, which were blue (λ450 nm), green (λ525 nm), yellow (λ590 nm), and white (λ400~780 nm), water temperature was controlled at (22±1)℃, photoperiod was 16L:8D. The light intensity was set at 200 mW/m2. The results showed that the fertilized eggs of Takifugu rubripes under the blue, green and yellow light hatched 2 days earlier than the white light. At the end of the experiment, the full length, body length, trunk length and tail length of the larvae under the blue light were the most. Under yellow light, the head length, eye diameter, and body height showed the fastest growth. For growth genes, the expression of growth hormone (GH) gene under the blue light was significantly higher than yellow and white light group (P<0.05), but there was no significant difference with green light group. There was no significant difference in gene expression for growth hormone receptor 1 (GHR1) and insulin-like growth factor type (IGF-1) under different spectra, but the expression of GH gene under blue light was the highest. The results showed that LED spectrum (blue, green, and yellow) promoted the hatching of the fertilized eggs of T. rubripes, and the blue spectrum is beneficial to the growth and development of the T. rubripes larvae. The paper provided a scientific reference for the incubation of T. rubripes.