Karyotype Analysis of Collichthys lucidus

Abstract:

In this study, the metaphase chromosomes of Collichthys lucidus were obtained from kidney tissue by the method of PHA and colchicine injection-air drying method. The experimental fishes were taken from Ningde coast of China. Because of their strong stress, we adjusted the drug treatment time (PHA: 6 h; Colchicine: 2 h, 5 h). In order to identify whether there were heteromorphic chromosomes, we separated the female from the male. The results showed that there were 48 chromosomes in C. lucidus and its karyotype formula was 2n=48t, NF=48. The relative length of chromosome was in the range of (5.694±0.514)~(3.039±0.161), which suggested that C. lucidus was in accordance with the original karyotype of perciformes and the higher group of fish evolutionary taxonomy. Sex chromosome, satellite chromosome and secondary constrictions were not found in C. lucidus. This study provides basic data for cytogenetics and germplasm identification for C. lucidus.