Abstract:There still exists considerable controversy regarding the taxonomic status of different geographical populations of Scapharca broughtonii in Northeast Asia. To study the taxonomic status of the Chinese population of S. broughtonii, the mitochondrial genomic COI and 12S rRNA sequences of the Chinese population of S. broughtonii were determined using PCR amplification and sequencing. To analyze the molecular phylogenetic relationships and classification, the homologous sequences of 26 species of shellfish were downloaded from GenBank, the molecular phylogenetic trees of COI and 12S rRNA of Arcidae molluscs were constructed using the neighbor-joining and maximum-parsimony methods with Mytilus edulis as the outgroup taxa. The results showed that the base composition, codon utilization, and mutation locus of COI and 12S rRNA genes were similar to those of the Japanese population of S. broughtonii and A. sativa, while the genetic distance was small, showing the genetic relationship was close. The classification status of the Chinese population of S. broughtonii was consistent with that of S. broughtonii. The purpose of this research was to better understand the arkshell genetic background and resources, to protect the natural resources, and promote the development of aquaculture.
