Abstract:Studies were conducted for further understanding the level of genetic diversity and population genetic structure of Fenneropenaeus chinensis under artificial breeding conditions. Fifteen fluorescence labeled microsatellite primers were used to analyze the genetic diversity and genetic differentiation in wild population (WP) and breeding population (BP) of F. chinensis. The wild population was collected from the western coast of Korean Peninsula (34°30¢N, 127°30¢E), and the breeding population was obtained after selection for successive ten generations. In each population, 40 samples were used for DNA extraction according to the protocol provided by the manufacturer. PCR was performed in a 25 µl reaction and the PCR products were sequenced by Sangon Biotech(Shanghai) Co., Led. Allele data was analyzed by Cervus 3.0.7 and GenALEx 6.502. The results showed that a total of 462 alleles were identified at 15 microsatellite loci, the numbers of alleles (Na) and effective alleles (Ne) were 3~44 and 2~29 in WP and BP, respectively. The polymorphism information content (PIC) ranged from 0.518 to 0.964, and the observed heterozygosity (Ho) values of WP and BP were 0.518 and 0.964, respectively. P-values were calculated to confirm whether the 15 loci deviated from the Hardy-Weinberg equilibrium or not. Of the 30 population loci, there were 11 population loci was heterozygote excess. The Shannon genetic diversity index (H) in WP and BP were 2.786 and 2.399, respectively. The Nei’s unbiased genetic Distance (uD), as well as unbiased genetic Identity (uI) was 0.17 and 0.838, respectively. The Gene Flow (Nm) and Fst value of the two populations were 17.997 and 0.017 (P=0.001), respectively, indicating that there was a low genetic differentiation. Partitioning of the genetic variation revealed that only 7.50% of the genetic variation was among the populations, and the other genetic variation was within the populations. This study showed that the ‘Huanghai No.2’ still has high genetic diversity after selection for successive ten generations, and it also has potential value for further breeding materials
