Genetic Analysis of 33 Microsatellite Loci in 3 Families of Exopalaemon carinicauda Inbred Line

Abstract:

To analyze the genetic effects and inbred degrees of 3 families of Exopalaemon carinicauda inbred line, 33 microsatellite loci were selected for genetic analysis. Composition of alleles of each line was analyzed. Number of allele (N), heterozygosity (H), polymorphism information content (PIC), and mean rate of homozygote were calculated. According to gene frequencies of 33 microsatellites, genetic distances between these lines were estimated. In additon, the dendrograms were obtained based on genetic distances. The results indicatedthat 72 alleles were detected at the 33 loci with an average number of allele of 2.758. The estimation of average N, H and PIC of the whole population was 2.758, 0.294, 0.272, respectively. The average N in three families were 2.258, 0.226, 0.214, respectively. The average H in three families were 2.469, 0.283, 0.300, respectively. The average PIC in three families were 2.219, 0.268, 0.207, respectively. And 7 loci could be used as special gene loci for genetic detection. Cluster analysis showed that Line A and B were gathered first, and then merged with Line H. In conclusion, this study revealed the pattern of genetic variation at 33 microsatellite loci in three inbred families of E. carinicauda. And the results indicated that inbred families have reached a certain degree of inbreeding. Some loci could be used as special gene loci for genetic detection and identification of independent genetic groups. This study provided important information for genetic analysis and germplasm conservation in E. carinicauda.