Comparison of the Body Color, Texture, Activities of Viscera Digestive Enzymes, Antioxidant Enzymes and ATP Enzymes of Two Cultured Population of Misgurnus anguillicaudatus

Abstract:

Misgurnus anguillicaudatus is a fish species with high economical values, and is widely distributed in China except for the Qinghai-Tibet Plateau. The popularity of this fish has been growing due to its high contents of minerals, amino acids and vitamins. Environment is one of the main factors that affect the body color, the texture and the visceral enzyme activities of the fish; hence it has been a hotspot in the research of M. anguillicaudatus. In this study we explored the living strategies of M. anguillicaudatus raised in two culture models, the pond monoculture (M1) and the polyculture of turtles, fish and shrimp in the rice field (M2). The wet earth from the paddy field served as the control (M0). We analyzed the differences in the body color, the texture, digestive enzymes, antioxidant enzymes and ATP enzymes between the two populations. The average body length and weight of the samples were (113.37±4.55) mm and (13.35±1.23) g for M1, and (122.69±5.22) mm and (16.43±1.36) g for M2. SPSS 19.0 and Excel 2003 were employed for data analysis. The results were as follows: (1) The measures of the dorsal chromatism of adult M. anguillicaudatus were M0>M1 (P<0.05). (2) There were significant differences in the measured indicators of the textures of dorsal muscles between the two populations, and all the values followed the order M2>M1 (P<0.05) except for that of adhesiveness. (3) There was no difference in the activities of the visceral digestive enzymes except for protease (M2>M1, P<0.05) and lipase (M1>M2, P<0.05). (4) Activities of antioxidant enzymes (CAT, SOD and POD) of M2 were higher than those of M1 (P<0.05). There were significant differences in activities of hepatopancreas enzymes between the two populations (P<0.05). The activities of Ca2+-Mg2+ATPase and Na+-K+ ATPase of M2 were significantly higher than those of M1 (P<0.05).