Studies on Culture and Storage of Gloiopeltis furcata Thalli

Abstract:

Three experiments were conducted in this study to explore proper on-land culture conditions for Gloiopeltis furcata thalli. In the first experiment vegetable fragments with different sizes (0.5cm, 1cm, and 1.5cm) and cut parts (cut from base, top, and fork) were incubated in lab for 60 days. In the second experiment G. furcata thalli were incubated in different containers (square tank, acrylic cylinder, and plastic membrane cylinder), and with different densities (0.5 g/L, 1 g/L, 2 g/L, and 4 g/L). In the third experiment G. furcata thalli dried in different ways (indoor wind, artificial climate incubation chambers, air-conditioning and fan) were stored at different temperatures (4℃ and –18℃). We found that with a certain size there was no significant difference in the growth between vegetable fragments from various cut parts. Vegetable fragments with a length of 0.5 cm grew slower than those with other lengths (1.0 cm and 1.5 cm, P<0.05). The SGR (specific growth rate) of G. furcata thalli was the lowest in the square tank (P<0.05), and was decreased along with the increase in culture density (P<0.05). The survival rate was higher in G. furcata thalli dried with air-conditioning and fan (P<0.05) and was 93.8% after preserved at –18℃ for 6 months. However, the survival rate of all groups decreased to 0 in 1 month after stored at 4℃. The results indicated that appropriate on-land culture conditions included reasonable size of vegetable fragments (> 0.5 cm), density between 0.5 g/L and 1 g/L, and plastic membrane cylinder as the container. Vegetable fragments for culture can be readily obtained if preserved at –18℃