Abstract:Entenmorpha sp. is widely distributed along the coast of China, particularly in the eastern regions, but it has not been utilized fully. To investigate the adsorption of glucose, cholesterol and nitrite to the dietary fiber extracted from Entenmorpha sp. by the method of complex enzymes (protease and cellulase), different ratios of protease to cellulase (5:1, 10:1, 15:1, 20:1, and 25:1) were used to extract dietary fiber from Entenmorpha sp. The results showed that the ratio of protease to cellulase in the complex enzymes had obvious effects on the adsorption capabilities of the dietary fiber. With the increased protease in the complex enzymes, the adsorption of glucose and cholesterol to the dietary fiber increased and then decreased. The dietary fiber using the 10:1 ratio of protease and cellulase had highest adsorption of glucose (20.03 mg/g). The dietary fiber using the 15:1 ratio of protease to cellulose had the highest adsorption of cholesterol (21.93 mg/g). With the increased protease, the adsorption of nitrite to the dietary fiber increased. The dietary fiber had highest adsorption of nitrite (29.25 µmol/g) at the 25:1 ratio of protease to cellulose. Furthermore, the adsorption capabilities of the diary fiber in the simulated gastric fluid at pH 2.0 and the intestinal fluid at pH 7.0 were investigated. The adsorption of cholesterol to the dietary fiber was higher in the simulated intestinal fluid than that in the simulated gastric fluid, while nitrite was the opposite. The adsorption of glucose to the dietary fiber had no significant difference in the simulated intestinal and the simulated gastric fluids. These results indicated that the adsorption of nitrite to dietary fiber happened in the stomach and cholesterol happened in the intestinal, and that the adsorption of glucose to the dietary fiber happened both in the stomach and in the intestinal.
