Optimized absorption method to isolate chondroitin sulfate from Oncorhynchus keta

Abstract:

The levels of chondroitin sulfate in Oncorhynchus keta are low, and the traditional method to separate and purify chondroitin sulfate primarily focus on cartilage. The present study was conducted to establish a new method to effectively obtain chondroitin sulfate from the low content raw materials. Chondroitin sulfate was prepared and isolated from the fish head through compound protease digestion and D958 resin adsorption. We also evaluated the effects of pH value of enzymatic hydrolysate, adsorption temperature and time on purification using the desorption capacity of chondroitin sulfate as an index. The results showed that the optimum purification conditions using D958 resin were as follows: the pH of enzymatic hydrolysate was 6.0, and the absorption temperature and time were 4℃ and 3 h respectively. The desorption rate was up to 87.66% under the optimum conditions. Chondroitin sulfate was effectively purified by the resin adsorption method. We have developed a key method to separate and purify chondroitin sulfate from low content raw material, which pave a new pathway for the utilization of wastes from O. keta processing.