Establishment of quantitative model on eel peripheral blood leukocytes phagocytosing Aeromonas hydrophila in vitro

Abstract:

In order to establish the quantitative model on eel peripheral blood leukocytes phagocytosing Aeromonas hydrophila in vitro, we first examined the variety of the number of bacteria phagocytosed by eel peripheral blood leukocytes and the survival ratio of the leukocytes when the infection time was unvariable and infection multiplicity was changed, as well as when infection multiplicity was stable and infection time changed. The results showed that the phagocytosis of eel peripheral blood leukocytes to A. hydrophila was significant, when the infection multiplicity was 100:1 and the infection time was 30 min, the number of the bacteria that phagocytosed by eel peripheral blood leukocytes reached saturation while the leukocytes still maintained high activity, indicating that 100:1 and 30 min was the optimal infection multiplicity and infection time. In addition, the quantity model was validated by detecting predation velocity and sterilization velocity. It was found that the phagocytosis velocity of eel peripheral blood leukocytes for A. hydrophila was faster than that for Escherichia coli, while the velocity for killing A. hydrophila was significantly lower than that for killing E.coli and Staphylococcus aureus, which shows that the quantity model established can be used for further study on intracellular survival mechanisms of A. hydrophila.