Abstract:Effect of waterborne Cd2+ at different concentrations (0.005mg/L, 0.025mg/L, 0.05mg/L, and 0.1mg/L) on activities of glutathione S-transferase (GST), acid phosphatase(ACP) and alkaline phosphatase (AKP) in digestive glands and gills of Sinonovacula constricta were studied. It was found that the GST activity in the two tissues was stimulated by Cd2+during the exposure time in all treatments, however, those in higher-dose treatments (0.05 mg/L,0.1 mg/L) decreased with the prolonged exposure. The activation of ACP by Cd2+ in the two tissues began at the 6h time-point, and the activity increased with the increasing concentration of Cd2+.For ACP activity, the higher-dose group reached the peak value earlier than the lower-dose group, and was inhibited after 72h. Except 0.005mg/L dose-treatment in digestive glands, the AKP activities in the two tissues showed a“decrease-increase-decrease”trend during the 168h exposure time in the experiment, and those in higher-dose treatments were stimulated earlier than those in lower ones. AKP activity in each treatment decreased constantly after reaching the peak value respectively. The fluctuations in GST, ACP, and AKP activities in the experiment indicated that exposure to lower concentrations of Cd2+ could have affected the metabolism and detoxification system of the organism.
