Abstract:The mRNA expression levels of HSP60 and HSP90 in tissues of gill, intestine, hepatopancreas, muscle, hemocyte and lymphoid were assayed by quantitative real-time PCR. Results showed that HSP60 and HSP90 were expressed in the six tissues. WSSV infection affected the transcription levels of HSPs mRNA. WSSV infection inhibited the expression of HSP60 in the tissues of gill, intestine, muscle and hemocyte, and promoted the expression of HSP90 in the tissues of gill, intestine, hepatopancreas, muscle, hemocyte and lymphoid. This indicated that both HSP60 and HSP90 took part in immune responses.