Abstract:Vibrio alginolyticus, V.harveyi and V.parahaemolyticus are main causes of ulcer disease for Pseudosciaena crocea. In this research, we set three experimental treatments of P. crocea which were injected with 0.2ml V. harveyi,V. alginolyticus or V. parahaemolyticus, respectively, while injection of normal saline at the same dose was given to the control.To study the impact of the three pathogenic Vibrios on the P. crocea’s blood biochemical parameters, contents of carbohydrate, lipid, protein, electrolyte and protein metabolism products and enzyme activity in serum were determined and compared by sampling at different time-point for 20d after infection. The results showed that the change of blood biochemical parameters occurred mainly in 1~4d after the Vibrio injection. Contents of TP, ALB, GLOB, ALT, AST, LDH, K,and Fe increased significantly(P<0.05), and contents of blood urea nitrogen, serum creatinine, blood sugar, cholesterol, Na,and Cl decreased significantly(P<0.05), while contents of TG, P, Ca, and Mg did not change significantly (P>0.05).These observations indicated that Vibiro infection has impacts on physiological functions and metabolic levels of P.crocea. The study indicates that the stress reaction to the Vibrios occurred mainly during the early stage after infection, and serum biochemical parameters can be used as early-warning indicators of ulcerous infection for P. crocea.
