Abstract:Experiments were carried out to explore the function of Chinese herbal medicine as immunostimulants in Scylla serrata. The formula included Astragalus membranaceus, Baicalensis Georgi, Eucommia ulmoides Oliver, and GLycyrrhiza uralensis Fisch in the proportion of 4∶3∶2∶1. The control group was injected with saline, while the experimental groups were injected with liquid formulated Chinese herbal formula medicine. The activity of enzymes in hemolymph firstly increased until the peak appeared 2-day after injection (except SOD), then decreased back to normal level 4-day after injection. The survival rate of treatment groups was improved to 53.33% when S. serrata was subjected to Vibrio parahaemolyticus. It is suggested that this herbal formula may be used as a herbal feed additive and serve as an effective immunostimulant in cultured aquatic animals.