Abstract:The residue of tetracycline antibiotics including oxytetracycline(OTC), tetracycline(TC), doxycycline(DC) and chlorotetracycline(CTC) in carps，eels, shrimps and sea cucumbers were determined with the optimized method of high performance liquid chromatography(HPLC) coupled with fluorescence detection(FL). The target compounds were extracted with acidic EDTA-Mcllvaine buffer solution,then enriched and cleared up using a hydrophilic-lipophilic balance(HLB) SPE cartridge,followed by separation by the C18 RP-HPLC column with imidazole buffer-methanol. The limit of quantification of tetracycline antibioitics in samples were 20, 20, 40, 40 μg/kg for TC, OTC, CTC and DC respectively. The average recovery for all the compounds were 70.5%~100%. The inter-variation coefficients were under 10.0%，and intra-variation coefficients were under 15.0%. The method is rapid, sensitive, accurate, reliable and can be applied in the analysis of antibiotics residues in aquatic products.
