Abstract:The metaphase chromosomes of Nibea albiflora(Richardson)and Nibea japonica were obtained from kidney tissue by the method of PHA and colchicines injection, and the karyotype of these two fishes were analyzed. The results showed that both fishes had 48 telocentric chromosomes in the diploid, and the karyotype formula was 2n=48t, NF=48. The relative length of chromosomes ranged from 3.06±0.20 to 5.94±0.33 in N. albiflora, and from 2.66±0.14 to 5.68±0.22 in N. japonica. Karyotype data of Sciaenidae species in previous reports were also collected including N. diacanthus, N. miichthioide and Pseudosciaena crocea. The clustering analysis was carried out for karyotype evolutionary distance among N. albiflora, N. japonica, N. diacanthus, N. miichthioide,and P. crocea. The results showed that the relationship between N. albiflora and N. diacanthus, and between N. japonica and N. miichthioide, were relatively close based on karyotype evolutionary distance, and the largest karyotype evolutionary distance (0064 8) were found between N. miichthioide and P. crocea. Results from the clustering analysis were consistent with the phylogeny based on the morphology of these species.
