Abstract:Seven microsatellite loci were identified in the GH, GHRH and PACAP genes of Cynoglossus semilaevis, and were used as polymorphic markers to analyze the genetic diversity and genetic structure of two wild populations (BS and YS) and a cultured population (HS). The results revealed that no genetic polymorphism was detected in three loci out of seven. The numbers of alleles in the other four loci ranged from 2 to 37, with an average of 9.5，and the numbers of effective alleles ranged from 2 to 28.9, with an average of 8.4. The observed and expected heterozygosities ranged from 0.514 5 to 0.773 8 and from 0.569 0 to 0.867 1, respectively. The average polymorphism information content per locus ranged from 0.482 9 to 0.831 4. The results of pairwise FST and individual distribution analysis demonstrated that there was no significant difference between the wild populations, while significant differences were observed between the wild and the cultured populations. Moreover, based on the distribution of alleles and diploid genotypes, no significant difference was observed between female and male in any of the three populations.
