Abstract:In this study, pEGFP-N1-ie1 was reconstructed by inserting WSSV immediate early gene, and vp28 was cloned in a new constructed vector. The survival rates and antiviral associated factors (Dicer, Argonaute, STAT and Lyzome) were detected in Litopenaeus vannamei after immunity by injection and oral-feeding. RT-PCR results showed that vp28 was detected in gill of shrimp by injection and oral-feeding immunity treatment. The relative protection rate of vp28 group reached 22.4% (injection group) and 36.84% (oral feeding group).The STAT and dicer mRNA expression in vp28 group were significantly higher than that of control group after injection treatment for 7 d. The argonaute mRNA expression in vp28 group was significantly higher than that of control group after injection treatment for 3 d and then decreased. The lysozyme mRNA expression in vp28 injection group was consistently significantly higher than that of control group. But the STAT and Argonaute mRNA expression in vp28 group was significantly higher than that of control group on the 7th day of oral-feeding treatment and then decreased. The dicer mRNA expression in vp28 group was significantly higher than that of control group on the 3rd day of oral treatment. These results demonstrated that vp28 DNA vaccine has a potential utility for control of WSSV disease in shrimp.
