Abstract:A multiplex polymerase chain reaction was developed for the simultaneous detection of five major foodborne pathogens, Vibrio parahaemolyticus,Staphylococcus aureus, Listeria monocytogenes,Salmonella enteritidi, and Shigella flexneri. Five pairs of specific primers were designed according to the thermolabile hemolysin gene of V. parahaemolyticus, the heat stable nuclease gene of S. aureus，the Listeriolysin O gene of L. monocytogenes, the invasion protein A gene of S. enteritidis, the invasion plasmid antigen H gene of S. flexneri. The reaction conditions were optimized, and the specificity and sensitivity of this method were tested.The detecting results of 48 samples also proved its reliability and practicability.
