Abstract:In order to reveal the effect of nocardiosis on Pseudosciaena croceas physiological conditions，the process of physiological and biochemical pathology was explored in this article. With the method of enzymology analysis, the change of enzyme activity in several main lesion tissues of P. croceas infected with Nocardia was tested. The results indicated that, in contrast with the control group, the amylase activities had no significant change in heart and spleen, while decreased apparently in kidney, liver and gill(P＜0.01).Lysozyme activities in heart, liver, kidney and gill increased significantly(P＜0.01), whereas declined significantly in spleen (P＜0.01). Alkaline phosphatase activities in heart, spleen, liver and gill increased significantly, while became lower in kidney (P＜0.01). Acidic phosphatase activities were higher in heart, spleen, and lower in kidney and liver,while no significant change in gill was found. Both glutamic-oxalacetic transaminease and glutamic-pyruvic transaminase increased significantly in kidney and decreased apparently in liver(P＜0.01), while none of them showed significant change in heart, spleen and gill(P＞0.05). Malondialdehyde both in kidney and liver decreased and had no significant changes in heart, spleen and gill. Superoxide dismutase activities reduced significantly in liver and gill(P＜0.01), while no significant changes in heart, spleen.The catalase activities reduced significantly in heart and kidney, and increased in spleen(P＜0.01), while no significant changes in liver or gill.Therefore, P. croceas had obvious stress response to Nocardia infection and showed different levels of damage to the organs of sicken fishes.
