Abstract:The gene fragments of mitochondrial DNA control region of 47 individuals of Sebastiscus marmoratus from eastern Guangdong coastal waters were amplified, sequenced and analyzed.By comparing with 10 corresponding D-loop gene sequences of Sebastidae from GenBank, the taxonomic status of Sebastidae was examined. Fragments (538~544 bp) of control region in mtDNA from 47 individuals of S.marmoratus were determined.A total of 69 variable nucleotide sites and 36 sites with gaps/missing were found and 38 haplotypes were defined. The relative amounts of A, T, C and G bases were 34.5 %，29.0 %，15.8 % and 20.7 %, respectively. The average number of haplotype diversity (Hd) and nucleotide diversity (Pi ) were 0.978 and 0.019 2，respectively. The molecular phylogenetic trees of Sebastidae constructed with H. armatus as outgroup showed that S. marmoratus was basal in the tree and differentiated earlier from their common ancestor. Sebastidae was monophyletic and 11 species were divided into four clades including genus Sebastes, Sebastiscus, Hozukius and Helicolenus, which was consistent with the traditional taxonomic results. In conclusion, mtDNA control region is suitable for the analysis of S.marmoratus not only in population genetic diversity but also in phylogenetic relationship.
