Abstract:Fish scale type I collagen was extracted through low temperature enzymolysis from Tilapia mossambica, and after being crosslinked with chitosan, hemostatic sponge was generated via freeze-drying. Through single factor comparative tests and orthogonal tests, the optimum preparation technique was obtained as follows: mixing of collagen with chitosan according to a given volume ratio 7∶3, maintaining total concentration of preparation mixture at 1.4%, and keeping dosage of cross-linking agent at 0.015%. Under this condition, the generated hemostatic sponge possessed excellent properties, such as density (22.95 mg/cm3), water absorption (45.34), water retention (55.83%), breathability (46.36%), and hemostatic time (68.0 s). A faveolate vesicular structure, which contributed to preferable properties, was clearly observed with SEM.
