Yeast surface display of white spot syndrome virus VP28

Abstract:

pYD1 was selected to make a vaccine using Saccharomyces cerevisiae pair as the live carrier. One pair of primers was designed according to the gene encoding white spot syndrome virus (WSSV) of VP28. VP28 gene was obtained by using PCR method. Fragment of VP28 was ligated into pYD1 and then transformed with E.coli TOP10. The construct was propagated in E. coli TOP10 and then was transformed into the yeast strain EBY100. The display of VP28 protein on the yeast surface was confirmed by fluorescent staining with antibody analysis. The results showed that VP28 was displayed on yeast surface. The infection test indicated that the yeast cells displaying VP28 was safe to Procambarus clarkii. This work is helpful for further research on the immunological effect of the live vaccine.