Abstract:The extraction of polysaccharides from Sargassum thunbergii by cellulase hydrolysis and ultrasound was studied. Effects of four factors including ultrasonic power, cellulaseconcentration,extraction time and extraction temperature were discussed. On the basis of single factor analysis, the optimal operation variables were described through orthogonal experiment. Results indicated that influence of factors on the extraction were in the following order: extraction time, extraction temperature, ultrasonic power, and cellulase concentration. The optimal condition for extraction were: 300 W ultrasonic power, 3% cellulase, 50 ℃ and 15 min. Moreover, the cellulase addition increased the extraction rate by 37.03% compared with the control group.
