he utmost length of PCR product in detection of white spot syndrome virus (WSSV) using dUTP

Abstract:

To prevent carry-over contamination, dTTP was replaced by dUTP in PCR detection for white spot syndrome virus (WSSV). Seven pairs of primers were designed according to the genome sequence of WSSV. The 600~1 800bp DNA fragments were amplified separately. It was found that the utmost length of PCR product is about 1 400bp, when dUTP is used to take place of dTTP.