Abstract:Two lectins with different molecular mass,designated as MSL-I and MSL-II, were isolated from serum of loach Misgurnus anguillicaudatus. The purification procedure consisted of separation on DEAE-Sepharose FF anion exchange column followed by gel filtration chromatography on Superdex 200 10/300 GL. Determination showed that MSL-I and MSL-II had a nature molecular weight of 25,000 and 82,000 respectively.The denatured SDS-PAGE showed that both MSL-I and MSL-II had two bands on the gel. We proposed that MSL-II was constituted with two subunits with molecular mass of 29,000 and 13,600 respectively. The subunit composition of MSL-I was to be further studied. Sugar-binding specificity test showed that glucose, D-galactose, D-fructose, D-xylose, L-arabinose and lactose had inhibitory effect on MSL-II, and among them lactose was most effective.Thermal stability test showed that MSL-II had a strong thermal stability even treated at 60℃ for 30min. The optimum pH of MSL-II was 6~8.
