Abstract:During Nov. 2008~Nov. 2010, 20 batches of turbot Scophthalmus maximus, stone flounder Kareius bicoloratus, and perch Perca fluviatilis were respectively collected from some coastal areas of Shandong Province, and the infection of red sea bream iridoviral disease (RSIVD) was investigated using PCR method recommended by the World Organization for Animal Health.The result showed that the RSIVD was detected in four samples. Based on the major capsid protein (MCP) gene of the two standard strains of red sea bream iridovirus and infectious spleen and kidney necrosis virus, degenerate primers were designed for PCR, and the MCP gene in the four positive samples were amplified and submitted to GenBank. The phylogenetic tree was drawn, and it was found that all of four isolated virus strains belonged to Megalocytivirus genus of Iridoviridae.
