Abstract:The crossbreed F1 progenies of Paralichthys dentatus♂×P. olivaceus♀ have been cultured since 2006 in indoor tanks. The F1 fish (27~33cm in total length) and their parents were randomly sampled for morphological measurements. There were no significant difference for morphological parameters between F1 fish and their parents. In addition, heterosis was manifested by ratio of body length and total height. The pigmentation and stripes on the body surface of F1 were different from their parents. Peripheral blood was sampled from F1 progenies and their parents respectively by withdrawing from tail vein. The DNA contents of erythrocytes in peripheral blood were determined using PARTEC Cell Counter Analyzer(CCA-Ⅱ). The DNA contents of F1 fish, P. dentatus and P. olivaceus were 1.51±0.02pg, 1.50±0.04pg and 1.43±0.16pg respectively. The DNA contents of F1 fish were similar to their male parents, P. dentatus, indicating that the heredity of F1 fish may bear more close relationship with P. dentatus. Meantime, the results showed that the F1 fish were diploid.
