Abstract:o determine the selection marker of N. oculata, the sensitivity to 5 antibiotics (Neomycin, Streptomycin, Hygromycin, Spectinomycin, and Zeocin) was determined in this study. For each antibiotic, liquid medium and solid medium were both adopted with the same concentration gradient. The concentration gradient of Neomycin and Streptomycin was 0, 100, 200, 400, 600, and 800μg/ml, while that of Hygromycin was 0, 25, 50, 100, 125, and 150μg/ml, and that of Spectinomycin was 0, 40, 80, 100, 150, and 200μg/ml. The concentrations of Zeocin were much lower, which were 0, 0.1, 0.2, 0.4, 0.5, and 1μg/ml. It was found that N. oculata is not sensitive to Neomycin, Streptomycin, Hygromycin or Spectinomycin, but highly sensitive to Zeocin. Zeocin can completely inhibit the growth of N. oculata when its concentration is 0.5μg/ml in solid medium or 1μg/ml in liquid medium. Zeocin could serve as a selection reagent in the genetic transformation of N. oculata, and the ble gene could be the selection marker.
