Abstract:The C-binding, Ag-NORs, and G-banding patterns of the metaphase chromosomes of barfin flounder Verasper moseri were investigated in this study. The results showed that the interstitial regions of most chromosomes exhibited positive C-bands, in which the whole 3rd, 13th, 20th, and 22nd pair of chromosomes showed totally positive C-band, whilst the 1st, 4th and 8th pair of chromosomes only showed positive C-bands in the middle region, the rest with centromeric positive C-bands. However, the 9th pair of chromosomes showed negative result. The C-banding heretochromatin occupied 54.91% of the total chromosome in the present study. Silver staining study showed that most chromosomes featured two silver-stained nuclear organizing regions (Ag-NORs) with the percentage of 87.5%. Accordingly, the interphase nucleus with 2 nucleolus appeared most frequently, thus it is supposed that the barfin flounder bears a pair of Ag-NORs which located at the end of short arm of the submetacentric chromosomes. A total of 59 positive G-bands (37 strong, 22 light) were recorded in 23 pairs of chromosomes. These results would provide basic data for the genomic research and biodiversity conservation of barfin flounder.
