Abstract:The single factor test and the orthogonal test results showed that the optimum alkali extraction process was: 2 volumes of 6% NaOH were added, followed by stirring in water bath at 40℃ for 4h. The orthogonal test results indicated that the optimum enzymolysis procedure was as follows: 0.5% pancreatin was added to the extraction solution adjusted to pH 8.6, and kept at 50℃ for 3h for hydrolysis, and then 0.3% pepsin was added to the solution adjusted to pH 5.7 and kept at 40℃ for 2h. The single factors test results of ethanol solution revealed that the best procedure was as follows: after adjusting pH of the obtained hydrolysate to 6.5, 95% ethanol was added to adjust the consistency of precipitation system to 70%. The quality of the product met the standard requirement of WS1-C3-0030-2000 (Chondroitin sulfate tablets) and WS-10001-(HD-0892)-2002 (Chondroitin sulfate injection).
