Rapid detection of Lactobacillus fermentum by PCR-DHPLC

Abstract:

Rapid detection of Lactobacillus fermentum was established by using polymerase chain reaction (PCR) and denaturing high-performance liquid chromatography (DHPLC). The primers were designed and the PCR system was optimized with the elongation factor (EF-Tu) of L. fermentum as the target gene. Twenty-six strains including L. gasseri were tested with specific detection. The sensitivity of various diluted standard strains were determined. The results indicated that the PCR-DHPLC method was specific and sensitive with a detection limit of 100 CFU/ml.