Abstract:We examined the population genetic structure of three families including parents, backcross, and inbreeding families of Japanese flounder using 10 microsatellite markers. The results showed that among the 10 loci, the allele number (NA) was 1.7~3.0, the average allele number was 2.3, the effective allele number (Ne) was 1.553 2~2.887 8, the average effective allele number was 2.0541, the observed heterozygosity (Ho) was 0.307 0~0.664 8, the average observed heterozygosity (Ho) was 0.487 0, the expected heterozygosity (He) was 0.200 0~0.606 8, the average expected heterozygosity (He) was 0.606 8, the average polymorphism information content (PIC) was 0.233 4~0.579 4, and the average polymorphism information content (PIC) was 0.399 5. The polymorphism information content of the three families were at the medium level. The value of PIC from the largest to the smallest was: parents family, backcross family, and inbreeding family. The Poli194TUF was single in the inbreeding family. The genetic deviation index was 0.067 1, which indicated that 6.71% deviation was from families and the genetic deviation was not high inter-families. The genetic distance obtained from the clustering analysis of the three families by UPGMA indicated that the parents family and the inbreeding family was closer. The allele purification was faster in inbreeding family, and thus it was suitable for strains establishment.
