Abstract:The antioxidant peptide ultraflexible nano-liposomes were prepared by using the thin-film dispersion method.The effects of some factors on the encapsulation efficiency,including the ratio of lecithin to cholesterol,temperature,concentration of the antioxidant peptide,rotation speed,pH,and ultrasonic emulsification time,were studied.The formulation was optimized by L9 (34) orthogonal design.The ultraflexible nano-liposomes were mostly unilamellar vesicles and their mean particle size was 255±60 nm.The optimum entrapment efficiency was 58.39%±0.34%.The antioxidant peptide liposome was stable at 4℃.Therefore,the antioxidant peptide ultraflexible nano-liposomes posses high encapsulation efficiency,good stability and reproducibility.
