Abstract:The complete sequences of mitochondrial control region of 53 individuals of Collichtys iucidus from seven populations, Nantong(NT), Qidong(QD), Luchaogang(LC), Wenzhou(WZ), Ruian(RA), Fuzhou(FZ), and Panyu(PY) were obtained by PCR cloning . Sequence lengths of the control region of C. iucidus varied in the range of 778-782 bp, which was mainly caused by the insertion or deletion. The conserved regions, such as TAS and CSB-1,-2, and -3 were identifiable in the control regions of C. iucidus. There were 66 polymorphic sites, which accounted for 8.44% in all sequences, and 33 haplotypes were found in all individuals. The average value of haplotype diversity (Hd) was 0.934 7±0.017 5 and the average value of nucleotide diversity (π) was 0.017 5±0.002 3 for seven populations. The K2-P distance within and among seven populations were 0.001 7～0.022 8 and 0.002 3～0.043 3, respectively. The difference index (FST) ranged betweenin -0.058 4～0.845 8 and the gene flow values were within the range of 0.05～19.62 for the seven populations. AMOVA analysis revealed that the inter-population variance was 74.21% and the intra-population variance was 25.79% (P<0.05). The phylogenetic tree estimated by NJ methods indicated that the C. iucidus individuals from seven populations could be clustered as two clades: the northern clade was formed by Nantong, Qidong, Luchaogang, and Wenzhou populations, while Fuzhou and Panyu popualtions formed the southern clade, and Ruian population was mixed with the above two clades.
