Abstract:Lipopolysaccharides (LPS) were extracted from ten Vibrio alginolyticus strains with different virulence. Five of them were virulent strains, named as HN08811, HN08155, HN08813, HN08335, and HN07006 and the others were non-virulent strains, named as HN08805, HN08307, HN08232, HN08201, and TG06003. The results of SDS-PAGE (sodium dodecyl sulfate polyacrylamide polyacrylamide gel electrophoresis) with 10 μg LPS showed that LPS of V. alginolyticus strains with different virulence had abundant polymorphism. The polymorphism of lipid A section of each LPS was difficult to distinguish. However, for the O-antigen section, 7~8 clear bands of LPSs from virulent strains were observed except for HN07006. LPSs from all the non-virulent strains had discernable slender and intensive bands. This method not only creates a new molecular typing method for rapid identification of virulent strains of V. alginolyticus, but also provides scientific theoretical data for the analysis of serotype of V. alginolyticus.
