Abstract:In this paper, chemotaxis of Vibrio harveyi TS-628 strain was studied by drop assay and capillary assay. The study of chemotaxis of V.harveyi to muscle extract, cutaneous mucus, intestinal mucus, serum of Epinephelus awoara showed that V. harveyi displayed stronger chemotaxis to muscle extract than to other tissues and the chemotaxis of V. harveyi was remarkably affected by temperature, pH and NaCl concentration. Furthermore, chemotaxis of V. harveyi to 18 kinds of amino acid of E. awoara muscle was studied, the results indicated that V. harveyi had strong chemotaxis to many kinds of amino acid and the most attractive amino acid was Val. However,the concentration of amino acid also affected the chemotaxis of V. harveyi.