Abstract:Artificial reef for Apostichopus japonicus cultivation were made of blue net, white gauze, green gauze, PE wove cloth, tiles and stones, and then various ecological factors were studies, including biomass of benthic algae, DO and nutrient salt of water, the locality and depth of artificial reef for Apostichopus japonicus cultivation in pond. The results are as follows:It was quite large that the biomass of benthic algae attached on dark-colored blue net and green gauze or when the water with a depth of less than 150cm; the biomass of small benthic algae was quite large on PE wove cloth and tiles; while large benthic diatom are likely adhesion on large hole net, for example blue net, white gauze and green gauze. High correlation was demonstrated between DO of bottom water and biomass of benthic algae, the coefficient being 0.8833. Although The NO3--N in the water was comparatively large that the coefficient of NO3--N was extremely small than NH4+-N of bottom water with biomass of benthic algae. The coefficient of NH4+-N of bottom water was comparatively large(0.7176) with biomass of benthic algae. Coefficient between PO43--P and biomass of benthic algae was the middle. Materials, setting way and water depth of artificial reef were key techniques and conditions culture Apostichopus japonicus in pond. It showed remarkable ecoeffects in ecosystem of a Apostichopus japonicus pond.