Study on immobilization of marine microbial esterase ETM-b with different material

Abstract:

Esterase from Bacillus sp. was immobilized with sodium alginate, gelatin and chitosan. The enzyme, immobilized with 2% chitosan, 1% glutaraldehyde and chitosan microspheres∶enzyme loading=4∶3 (g/ml) exhibited the highest activity recovery (66%). The immobilized esterase with chitosan could be reused for 10 times, and 70% of enzyme activity remained after the 10th batch, which exhibited good operational stability. The immobilized esterase can catalyze α-naphthyl acetate in non-aqueous system, and it displayed high activity in isooctane, n-octane and n-hexane.